Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells

Table 1 Primers used in the different experiments of this study

Gene	Sequence (5´-3´)		Amplicon (pb)	Application
SOST	Forward	ATGCCACGGAAATCATCCCC	185	Expression in vascular tissue. Check the efficiency of cells transduction. Quantify the expression under different conditions in VSMCs in vitro.
SOST	Reverse	GTCACGTAGCGGGTGAAGTG	185
RPL13	Forward	CGTAAGATCCGCAGACGTAAGGC	228	Constitutive gene
RPL13	Reverse	GGACTTGTTCCGCCTCCTCGGAT	228	Constitutive gene
ALPL	Forward	GGCTGGAGATGGACAAGTTC	152	Quantify the expression under different conditions in VSMCs in vitro.
ALPL	Reverse	ACGCTCAGTGGCTGCGCTTA	152
RUNX2	Forward	CGCCGTGGTCCTATGACCAGTCTTA	169	Quantify the expression under different conditions in VSMCs in vitro.
RUNX2	Reverse	AGGCAGAAGTCAGAGGTGGCAGTGT	169
IL1β	Forward	CTTCAGGCAGGCCGCGTCAGTTGTT	202	Quantify the expression under different conditions in VSMCs in vitro.
IL1β	Reverse	CCGGAGCGTGCAGTTCAGTGATCGT	202
IL6	Forward	AGACAGCCACTCACCTCTTCAGAAC	208	Quantify the expression under different conditions in VSMCs in vitro.
IL6	Reverse	CCAGGCAAGTCTCCTCATTGAATCC	208
IL8	Forward	GAGAGTGATTGAGAGTGGACCAC	112	Quantify the expression under different conditions in VSMCs in vitro.
IL8	Reverse	CACAACCCTCTGCACCCAGTTT	112
COX2	Forward	CCGCCATCATCCTAGTCCTCATCGC	143	Quantify the expression under different conditions in VSMCs in vitro.
COX2	Reverse	TAGTCCGCCGTAGTCGGTGTACTCG	143
ACTA2	Forward	CATCGTGCTGGACTCTGGAGATGGT	151	Quantify the expression under different conditions in VSMCs in vitro.
ACTA2	Reverse	GAAGGAATAGCCACGCTCAGTCAGG	151

SOST, sclerostin; RPL13, ribosomal protein L13; ALPL, alkaline phosphatase, biomineralization associated; RUNX2, runt-related transcription factor 2; IL1β, interleukin 1 beta; IL6, interleukin 6; IL8, interleukin 8; COX2, cyclooxygenase 2; ACTA2, actin aortic smooth muscle; VSMCs, vascular smooth muscle cells.

ISSN: 1475-2840