Fig. 6

Effect of sclerostin overexpression on proliferation and apoptosis in HAoSMCs. (A) Effect of sclerostin overexpression on proliferation in HAoSMCs compared to mock (n = 4 biological replicates in each time/group). Each result was expressed as the OD at 570 nm. (B) Percentage of apoptosis in HAoSMCs with sclerostin overexpression compared to mock (n = 3 biological replicates/group and n = 2 technical replicates/ biological replicates). Data represent as the mean ± standard deviation of experiments performed. The p-values between groups were determined by the unpaired Student´s t-test. * = p < 0.05. HAoSMCs, Primary Human Aortic Smooth Muscle Cells; OD, optical density.