Table 2 Primer sequences and thermocycler settings for RT-qPCR
Target (product size) | Primer | Sequence (5ʹ–3ʹ) | Temperature profiles | |
|---|---|---|---|---|
ACTB (215) | Forward Reverse | CCTCTATGCCAACACAGTGC CATCGTACTCCTGCTTGCTG | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 84 °C for 1 s |
CDH5 (152) | Forward Reverse | CTACCAGCCCAAAGTGTGTG GTGTTATCGTGATTATCCGTGA | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 82 °C for 1 s |
COL1A1 (140) | Forward Reverse | GAGGGCCAAGACGAAGACATC CAGATCACGTCATCGCACAAC | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 84 °C for 1 s |
IL6 (149) | Forward Reverse | GGGGCTGCTCCTGGTGTTG CTGAGATGCCGTCGAGGATGTA | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 80 °C for 1 s |
MALAT1 (119) | Forward Reverse | TCTTAGAGGGTGGGCTTTTGTT CTGCATCTAGGCCATCATACTG | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 80 °C for 1 s |
PECAM1 (158) | Forward Reverse | AGACAACCCCACTGAAGACGTCG CCTCTCCAGACTCCACCACCTTAC | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 82 °C for 1 s |
TAGLN (191) | Forward Reverse | GGCAGGCCCCAGTAAAGAAG TGCCAGCCCACCCAGATT | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 84 °C for 1 s |
ZFAS1 (91) | Forward Reverse | CAGCGGGTACAGAATGGA TCAGGAGATCGAAGGTTGTAGA | Denaturation Annealing Extension Signal | 95 °C for 5 s 55 °C for 10 s 72 °C for 15 s 82 °C for 1 s |