Skip to main content
Fig. 3 | Cardiovascular Diabetology

Fig. 3

From: Non-coding RNA-mediated endothelial-to-mesenchymal transition in human diabetic cardiomyopathy, potential regulation by DNA methylation

Fig. 3

Changes in promotor methylation of ZFAS1, miR-9, and miR-200b in the heart in diabetes. Methylation of A ZFAS1 and B miR-9 promoters decreased significantly in the heart in diabetes compared to non-diabetic ones, but ZFAS1 promoter maintained an average M/U ratio of greater than 1 (denoted by the dashed line), whereas miR-9 promoter M/U ratio was less than 1 in both non-diabetic and diabetic cohorts. Methylation of C miR-200b promoter was significantly increased, with the M/U ratio changing from < 1 to > 1. [lncRNA expressions normalized to ACTB mRNA; miRNA expressions normalized to U6 small nuclear RNA; n = 10 for non-diabetic, n = 28 for diabetic; data presented as scatter plots with mean ± standard deviation; * = p < 0.05, ** = p < 0.01 as determined by Student’s T-test]

Back to article page

Cardiovascular Diabetology

ISSN: 1475-2840