Fig. 5

Inactivation of IGF-1R modulated by Klotho inhibited renal ox-LDL deposition. A, B The IGF-1R expression was investigated by IF analysis using Nephrin and WT1 in the kidneys of DKD patients and mice, compared to HC and WT groups. C IF analysis using WT1 and Nephrin was conducted to evaluate the impact of Klotho on IGF-1R expression in podocytes of WT DKD, KL^+/− DKD and TgKL DKD, respectively. D Western blot analysis was used to identify the levels of the protein IGF-1R in high glucose-treated podocytes, in the absence or presence of Klotho. E IF analysis of podocytic ox-LDL deposition by administration of PPP and IGF2 on KL^+/− DKD and TgKL DKD, respectively. *P < 0.05; **P < 0.01; ***P < 0.001