Fig. 5
From: Dual roles of myocardial mitochondrial AKT on diabetic cardiomyopathy and whole body metabolism
Activation of myocardial mitochondrial AKT1 improved whole body metabolism in diabetes. A Representative transverse PET images of differential heart muscle uptake/retention of [18F] Fluoro-4-Thia-Oleate (FTO). CAMCAKT mice placed on 2 months of HFFD after TAM or CO injection were administered 7.4 MBq [18F]FTO followed by a 35 min dynamic scan. B Organ biodistribution (%ID/gram) of [18F]FTO in CAMCAKT after 2 months of HFFD following TAM or CO injection. Mice were administered 7.4 MBq [18F]FTO followed by tissue collection after 40 min (n = 7 (CO), n = 6 (TAM); p < 0.0001). C Metabolic cage analysis was performed on CAMCAKT mice after 2 months of HFFD following TAM or CO injection. Kinetic data for oxygen consumption (n = 7 each group, p < 0.01, < 0.05) and D carbon dioxide production are shown as the mean for each time point and summarized as means for light and dark periods (n = 7 each group, p < 0.01, < 0.05). E. Energy expenditure was calculated from metabolic cage measurements (n = 7 each group, p < 0.01, < 0.05). F Body composition measurements of CAMCAKT after 2 months of HFFD following TAM or CO injection (n = 7 (CO–normal chow), n = 10 (CO), n = 10 (TAM); p < 0.0001). G Left, representative images of H&E-stained liver sections showed reduced liver steatosis in TAM-CAMCAKT mice after 2 months of HFFD. Right, lipid droplet percent area and size were quantified. Scale bar = 100 μm (n = 9 each group; p < 0.001, 0.01). H Liver uptake (%ID/organ) of [18F]FTO in CAMCAKT mice after 2 months of HFFD following TAM or CO injection. Mice were administered 7.4 MBq [18F]FTO followed by tissue collection after 40 min (n = 7 (CO), n = 6 (TAM); p < 0.01)