Table 2 Primary antibodies used in western blot and immunofluorescent staining
Name | Manufacturer | Cat No. | Dilution | Host | Application |
|---|---|---|---|---|---|
MICU1 | CST | 12524s | 1:1000 | Rabbit | WB |
MICU2 | Abcam | Ab101465 | 1:1000 | Rabbit | WB |
MCU | Abcam | ab219827 | 1:500 | Mouse | WB |
MCUR1 | Boster | A08547-1 | 1:1000 | Rabbit | WB |
p-eNOS | Abclonal | AP0421 | 1:1000 | Rabbit | WB |
iNOS | Abclonal | A0312 | 1:1000 | Rabbit | WB |
Caspase 1 | Immunoway | YT5743 | 1:1000 | Rabbit | WB |
NF-κB | Abclonal | AP0475 | 1:1000 | Rabbit | WB |
NLRP3 | Abclonal | A5652 | 1:1000 | Rabbit | WB |
IL-1β | Abclonal | A16288 | 1:1000 | Rabbit | WB |
eNOS | Abclonal | A15075 | 1:1000 | Rabbit | WB |
Cleaved caspase 1 | CST | 89,332Â S | 1:1000 | Rabbit | WB |
TNF-α | Abclonal | A11534 | 1:1000 | Rabbit | WB |
β-actin | Abclonal | AC026 | 1:5000 | Rabbit | WB |
CD31 | Abcam | ab7388 | 1:400 | Rat | IHC-Fr, ICC/IF |
VE-cadherin | Abcam | Ab205336 | 1:500 | Rabbit | ICC/IF |
ICAM-1 | Abclonal | A5597 | 1:100 | Rabbit | ICC/IF |
Donkey anti-Rat AF488 | Thermo Fisher | A21208 | 1:500 | Donkey | ICC/IF |
Donkey anti-Rat AF594 | Thermo Fisher | A21209 | 1:500 | Donkey | IHC-Fr, ICC/IF |
Donkey anti-Rabbit AF594 | Thermo Fisher | A21207 | 1:500 | Donkey | IHC-Fr, ICC/IF |