Table 1 PCR primers
Gene Name | Forward Primer | Reverse Primer |
|---|---|---|
MICU1loxp/loxp | GAACTAGCCAAGATAAACAGCGTG | GTGTGTTTCCTGTGGTGGTCTG |
CDH5-cre | CCAGGCTGACCAAGCTGAG | CCTGGCGATCCCTGAACA |
iNOS | TGGAGCCAGTTGTGGATTGTC | GGTCGTAATGTCCAGGAAGTAG |
IL-1β | CAGCACATCAACAAGAGCTTCAG | GAGGATGGGCTCTTCTTCAAAGA |
TNF-α | GACCCTCACACTCAGATCATCTT | CCTTGAAGAGAACCTGGGAGTAG |
ICAM-1 | AAGGGCTGGCATTGTTCTCTAAT | GCCTCCCAGCTCCAGGTATAT |
NF-κB | CTTCTGGGCCTTATGTGGAGATC | GGTCCTGTGTAGCCATTGATCTT |
NLRP3 | AGATGGAGAAGGCAGATCACTTG | CAAATGGAGATGCGGGAGAGATA |
MICU1 | ACAACAGTCCTCTCCACTCCTCAG | CCTCCATGTCTACCTCTCCGTCTC |
β-actin | AGCCATGTACGTAGCCATCCA | TCTCCGGAGTCCATCACAATG |