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Fig. 7 | Cardiovascular Diabetology

Fig. 7

From: Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injury

Fig. 7

Cardiac endothelial-specific overexpression of MICU1 alleviates diabetes-induced cardiac microvascular injury in diabetic mice. (A) A TUNEL assay was performed to assess CMECs apoptosis, scale bar = 20 μm. (B) Percentages of TUNEL-positive nuclei over total number of nuclei (n = 6 per group). (C) Percentages of TUNEL positive CMECs over total number of CD31-positive nuclei (n = 6 per group). (D) Cardiac microvascular perfusion is indicated by lectin-positive microvessels (green) to CD31-positive microvessels (red), scale bar = 20 μm. (E) Quantification of Lectin positive microvessels is shown (n = 6 per group). (F) Immunofluorescence staining of VE-cadherin was performed to observe changes in cardiac microvessel endothelial integrity and barrier function, scale bar = 50 μm. (G) Comparative quantitative analysis of fluorescence intensity of VE-cadherin (n = 6 per group). (H) Representative fluorescence images of intercellular adhesion molecule ICAM-1, scale bar = 50 μm. (I) Comparative quantitative analysis of fluorescence intensity of ICAM-1 (n = 6 per group). **p < 0.01

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Cardiovascular Diabetology

ISSN: 1475-2840