Fig. 2
Endothelial-specific knockout of MICU1 aggravates diabetes-induced cardiac dysfunction in mice. (A) Western blotting for protein levels of MICU1 in CMECs of WT and MICU1ecKO mice (aged 18 weeks). (B) The heart weight of different groups. (C) The ratios of heart weight to tibia length (HW/TL) for different groups. (D) Representative echocardiographic images of the LV in different 18-week-old mice. (E, F, G and H) Echocardiographic assessment of heart rate (E), Echocardiographic assessment of LV fractional shortening (LVFS; F), LV end diastolic diameter (LVIDd; G) and LV diastolic posterior wall thickness (LVPWd; H) in different mice (n = 6, 18 weeks old per group). (I) Cardiomyocyte cross-sectional area was analyzed using hematoxylin and eosin staining and wheat germ agglutinin staining in hearts from different mice (n = 6, 18 weeks old per group); scale bar = 20 μm. (J) Comparative quantitative analysis of cardiomyocyte CSA. (K) Representative images and comparative quantitative analysis of Masson trichrome staining of hearts from different mice (n = 6, 18 weeks old per group); scale bar = 20 μm. *p < 0.05, **p < 0.01