Fig. 1

MICU1 expression is decreased in CMECs of diabetic mice. (A) Cell type-specific MICU1 mRNA expression in different organ tissues and hearts of adults. The MICU1 mRNA levels in different organ tissues and hearts of adults were determined by mining single-cell RNAseq data deposited in the Human Protein Atlas database (https://www.proteinatlas.org/ENSG00000107745-MICU1/single+cell+type/heart+muscle). Different cardiac cell types are colored according to clusters and expressed by uniform manifold approximation and projection. (B) qRT-PCR analysis of MICU1 mRNA levels in the CMECs of diabetic db/db mice and wild-type littermate mice (n = 6, 6-, 12- and 18-week-old mice per group). (C) Western blotting analysis of the protein expression levels of MICU1 and β-actin in the CMECs of diabetic db/db mice and wild-type littermate mice (n = 6, 6-, 12- and 18-week-old per group). (D) qRT-PCR analysis of MICU1 mRNA levels in the CMECs treated with HGHF (n = 3 per group). (E) Western blotting analysis of MICU1 expression in CMECs treated with HGHF (n = 3 per group), **p < 0.01