Fig. 2

Immune cell profiling in WAT SVF of cohort 1

(a) Bubble plots representing the fold changes in the frequencies of WAT SVF immune cell populations for the three metabolic diseases groups CVD (n = 10), T2D (n = 10) and CVD + T2D (n = 10) compared to healthy controls (n = 9). Size of the circles corresponds to the strength of the p-values that were calculated by one-way ANOVA with multiple comparisons. 1-log10(p-value) > 2.3 are considered significant. The color of the circles corresponds to the mean fold change in the frequencies of each immune cell population in the disease group compared to healthy controls. The percentage of M1 (CD206⁺CD11c⁺) (b) and M2 (CD206⁺CD11c⁻) (c) macrophages in total WAT macrophage population (CD14⁺CD206⁺), M1/M2 ratio (d), the percentage of total CD3⁺ T (e) cells and CD4⁺ T cells (f) (in total immune cell population (CD45⁺). Grey, green, yellow, and blue bars represent healthy, CVD, T2D and CVD + T2D respectively. One-way ANOVA with multiple comparisons was used in b to f. * p < 0.05 and ** p < 0.01. Cohort 1 was used in (a) - (f).