Fig. 8
From: Insulin treatment increases myocardial ceramide accumulation and disrupts cardiometabolic function
Chronic insulin injections disrupt myocardial mitochondrial function. 16-week-old male mice received injections of PBS (daily), insulin (INS; daily; 0.75 mg/kg), myriocin (MYR, thrice weekly; 3 mg/kg), or INS + MYR. Heart samples were processed for imaging via electron microscopy (a) and quantified based on average greatest mitochondrial diameter (b; n = 3). A portion of samples was used to probe for mitochondrial complexes (c; n = 3) and Drp1 (D; n = 3). *P < 0.05 for condition vs. PBS