Fig. 3
From: Insulin treatment increases myocardial ceramide accumulation and disrupts cardiometabolic function
Insulin treatment affects cardiomyocyte mitochondrial physiology. H9C2 cardiomyocytes were treated with insulin (INS; 50 nM) with or without myriocin (MYR; 10 μM), an inhibitor of ceramide biosynthesis, for the times indicated. Following treatment, cells were imaged to determine mitochondrial morphology (a; n = 3), and analyzed for Drp1 protein levels (b; n = 4), and mitochondrial complex proteins (c; n = 4). *P < 0.05 for INS vs. PBS; #P < 0.05 for INS + MYR vs. INS alone