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Figure 4 | Cardiovascular Diabetology

Figure 4

From: Protein kinase RNA- like endoplasmic reticulum kinase (PERK) signaling pathway plays a major role in reactive oxygen species (ROS)- mediated endoplasmic reticulum stress- induced apoptosis in diabetic cardiomyopathy

Figure 4

Detection of ER stress and its sensors in cardiac tissue in different groups. (a) Immunoblots of GRP78 and ER stress sensors in different groups. The left side of this figure shows immunoblots of GRP78, p-PERK, PERK, p-IRE1, IRE1, cleaved ATF6 and beta-actin from top to bottom respectively in Ctrl, NAC, DCM and DCM + NAC. The right side of this figure demonstrates quantitative analysis of immunoblots on the left side of this figure. p-PERK/PERK ratio, p-IRE1/IRE1 ratio are used to stand for activation levels of PERK and IRE1; expression levels of GRP78 and cleaved ATF6 which are normalized to beta- actin are used to stand for activation levels of GRP78 and ATF6 among different groups. (b) Immunoblots and real- time quantitative PCR analysis of activation of PERK branch of ER stress in different groups. The left part of this figure shows immunoblots of ATF4, CHOP and beta-actin in Ctrl, NAC, DCM and DCM + NAC respectively. The middle part of this figure shows real-time PCR analysis of mRNA expression of ATF4 and CHOP (normalized to beta- actin) in Ctrl, NAC, DCM and DCM + NAC groups respectively. The right part of this figure shows quantitative analysis of immunoblots of ATF4 and CHOP (normalized to beta- actin) in Ctrl, NAC, DCM and DCM + NAC groups respectively. Values in this figure are presented by columns in (mean ± SD) manner. a Values are significantly different from Ctrl; b values are significantly different from NAC; c values are significantly different from DCM. [(&)P < 0.05; (#)P < 0.01; (*)P < 0.001].

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