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Table 2 Pharmacological parameters of vasomotor responses to phenilephrine ( PE), acetylcholine ( Ach), sodium nitroprusside ( SNP) and asymmetric NG, NG-dimethyl-L-arginine ( ADMA) in isolated aorta

From: Effect of silibinin on endothelial dysfunction and ADMA levels in obese diabetic mice

PE

 

pD2

EC50 (nmol/L)

Emax (% K+)

db/m + vehicle

7.27 ± 0.11

53.7 (32.1-89.7)

126.0 ± 7.0

db/db + vehicle

7.74 ± 0.10**

18.3 (11.3-29.9)

122.3 ± 5.0

db/db + silibinin

7.66 ± 0.08**

21.7 (15.3-30.7)

122.6 ± 3.7

ADMA

 

pD 2

EC 50 ( μmol/L)

E max (% K + )

db/m + vehicle

3.36 ± 1.76

440 (0.1-106)

65.6 (± 154)

db/db + vehicle

4.48 ± 0.36**

32.8 (5.8-188)

69.6 ± 12.0

db/db + silibinin

4.51 ± 0.40**

30.1 (4.8-194)

71.2 ± 12.6

ACh

 

pD 2

EC 50 (nmol/L)

E max (% tone)

db/m + vehicle

7.54 ± 0.12

28.8 (16.7-49.5)

35.7 ± 2.5

db/db + vehicle

6.74 ± 0.15**

183 (90.2-372)

62.1 ± 2.1**

db/db + silibinin

6.89 ± 0.12**

130 (74.9-224)

43.7 ± 2.8††

SNP

 

pD 2

EC 50 (nmol/L)

E max (% tone)

db/m + vehicle

7.97 ± 0.04

10.1 (8.8-12.9)

9.4 ± 1.0

db/db + vehicle

7.45 ± 0.06**

35.7 (26.5-48.0)

16.7 ± 1.7*

db/db + silibinin

7.56 ± 0.05**

27.7 (21.7-35.3)

11.8 ± 1.5

  1. Pharmacological parameters are from non linear regression; pD2 is the negative logarithm of the concentration producing 50% of the maximum effect, EC50 is the concentration producing 50% of maximum effect (95% confidence limits are given in parenthesis), Emax is the maximum effect (vasoconstriction to PE or ADMA in % of vasoconstriction evoked in the same preparations by 100 mmol/L K+; vasodilatation to ACh or SNP as % of residual tone). Statistical comparisons were made on the whole curves by two-way ANOVA; n = 12 preparations from 6 mice in each group; *P < 0.05, **P < 0.01 versus db/m + vehicle, ††P < 0.01 versus db/db + vehicle.
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Cardiovascular Diabetology

ISSN: 1475-2840